猪源氟喹诺酮耐药大肠杆菌通过接合水平传递耐药性的研究

doi:10.11843/j.issn.0366-6964.2016.04.021

Abstract

Abstract:

In order to investigate the role of mobile plasmids in the horizontal transmission mechanism of drug resistance in Escherichia coli isolated from pigs，the conjugation experiments of fluoroquinolone resistance and PMQR genes positive Escherichia coli were carried out，the minimum inhibitory concentration(MIC) of 8 kinds of common drug in transconjugants were figured by using broth micro dilution method and the plasmid mediated quinolone resistance gene(PMQR) were detected by PCR methods with specific primers designed after the conjugation experiments.The results showed that 16 strains of bacteria were joined successfully within 41 strains of fluoroquinolone resistance and PMQR genes positive donors，successful rate of conjugation experiments was up to 39%，transconjugants showed kinds of resistant phenotypes compared with J53 strain through testing the figure of MIC values，and compared with donor strains，87.5% of the transconjugants reveals changes of drug resistance patterns and the phenomenon of missing a drug resistance with obtaining another drug resistance，the results of PCR showed the decline of gene phenotypes compared with donor strains，qnrS gene revealed the highest successful conjugation rate，the genes of oqxA and oqxB can transferred in the same mobile plasmid，12.5% of the transconjugants displayed that the genes of oqxA，oqxB and qnrS can be transferred by the same mobile plasmid.The results above suggest that different PMQR genes show different success rates in the process of conjugation jointing with mobile plasmid which mediated resistance genes transferred horizontally in strains，which suggest the different PMQR genes may be located in the different mobile plasmid，by comparing the changes of drug resistance and gene phenotypes before and after conjugation experiments，especially the detection rate of PMQR gene，it can be preliminarily confirmed that the mobile plasmids play a very important role in the process that antimicrobial agents resistance genes are transferred horizontally in Escherichia coli strains.

CLC Number:

S852.612

WANG Hong-dong，XU Guo-feng，JIAO Wei-wei，ZHANG Xiu-ying. Investigation of Antimicrobial Agents Resistance Transferred Horizontally in Fluoroquinolone Resistant Escherichia coli from Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 805-811.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.04.021

https://www.xmsyxb.com/EN/Y2016/V47/I4/805

References

［1］潘渭涓，陈祥，王晓泉，等.1993-2008年禽源大肠杆菌和沙门菌对喹诺酮类药物耐药性分析［J］.中国人兽共患病学报，2009，25(7)：630-635.
PAN W J，CHEN X，WANG X Q，et al.The analysis of quinolone resistance of the avian Escherichia coli and Salmonella isolates from 1993 to 2008［J］.Chinese Journal of Zoonoses，2009，25(7)：630-635.(in Chinese)
［2］CLEWELL D B，AN F Y，WHITE B A，et al.Sex pheromones and plasmid transfer in Streptococcus faecalis：a pheromone，cAM373，which is also excreted by Staphylococcus aureus［J］.Basic Life Sci，1985，30：489-503.
［3］MARTÍNEZ-MARTÍNEZ L，PASCUAL A，JACOBY G A.Quinolone resistance from a transferable plasmid［J］. Lancet，1998，351(9105)：797-799.
［4］张辉，杨振泉，赵隽，等.大肠杆菌 ERIC-PCR分子分型方法的建立及其初步应用［J］.江苏农业学报，2010，26(5)：1098-1103.
ZHANG H，YANG Z Q，ZHAO J，et al.Generating of ERIC-PCR molecular typing method and its application in Escherichia coli［J］.Jiangsu Journal of Agricultural Sciences，2010，26(5)：1098-1103.(in Chinese)
［5］WANG M，GUO Q，XU X，et al.New plasmid-mediated quinolone resistance gene，qnrC，found in a clinical isolate of Proteus mirabilis［J］. Antimicrob Agents Chemother，2009，53(5)：1892-1897.
［6］JOHN P H，RENATER R，TAKASHIA，et al.M49-P methods for broth dilution susceptibility testing of bacterial isolated from aquatic animals;proposed guideline［S］.Pennsylvania:Clinical and laboratory Standards Institute，2005.
［7］YOSHIDA H，BOGAKI M，NAKAMURA M，et al.Quinolone resistance-determining region in the DNA gyrase gyrA gene of Escherichia coli［J］.Antimicrob Agents Chemother，1990，34(6)：1271-1272.
［8］朱恒乾，廖晓萍，陈朝喜，等.宠物源大肠杆菌质粒介导喹诺酮类耐药基因流行性检测［J］.中国农业科学，2010，43(16)：3447-3454.
ZHU H Q，LIAO X P，CHEN C X，et al.Detection of plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from pet animals［J］.Scientia Agricultura Sinica，2010，43(16)：3447-3454.(in Chinese)
［9］安微，张秀英，徐国锋，等.猪源多重耐药大肠杆菌通过接合水平传播传递耐药性研究［J］.中国兽医杂志，2014，50(5)：76-78，81.
AN W，ZHANG X Y，XU G F，et al.Study of antibiotic resistance transferred horizontally in multidrug-resistant Escherichia coli from swine［J］. Chinese Journal of Veterinary Medicine，2014，50(5)：76-78，81.(in Chinese)
［10］CAVACO L M，HANSEN D S，FRIIS-MØLLER A，et al.First detection of plasmid-mediated quinolone resistance(qnrA and qnrS) in Escherichia coli strains isolated from humans in Scandinavia［J］.J Antimicrob Chemother，2007，59(4)：804-805.
［11］王明贵，TRAN J H，JACOBY G A，等.大肠埃希菌临床分离株对喹诺酮类抗菌药的质粒介导耐药［J］.中国感染与化疗杂志，2006，6(4)：217-221.
WANG M G，TRAN J H，JACOBY G A，et al.Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli［J］.Chinese Journal of Infection and Chemotherapy，2006，6(4)：217-221.(in Chinese)
［12］刘渠，刘衡川，白松涛，等.食品中大肠埃希氏菌、沙门氏菌整合子的耐药性水平传递研究［J］.现代预防医学，2004，31(5)：681-684.
LIU Q，LIU H C，BAI S T，et al.Antibiotic resistance horizontal transfer of integrons in Escherichia coli and Salmonella spp.strains isolated from food［J］.Modern Preventive Medicine，2004，31(5)：681-684.(in Chinese)
［13］李德喜，刘建华，张素梅，等.猪源大肠杆菌质粒和染色体介导的喹诺酮类药的耐药机制［J］.中国兽医学报，2011，31(9)：1262-1265.
LI D X，LIU J H，ZHANG S M，et al.Mechanism of plasmid- and chromosome-mediated quinolone resistance in Escherichia coli isolated from pigs［J］.Chinese Journal of Veterinary Science，2011，31(9)：1262-1265.(in Chinese)
［14］代敏，王雄清，殷桂兰.四环素耐药基因的生化和遗传机制研究进展［J］.绵阳师范学院学报，2006，25(5)：72-78.
DAI M，WANG X Q，YIN G L.Advances of the research in biochemical and genetic mechanism of tetracycline resistance gene［J］.Journal of Mianyang Normal University， 2006，25(5)：72-78.(in Chinese)
［15］田哲，张昱，杨敏，等.四环素类药物酶修饰基因-tet(X)的起源、分布及在环境中的作用［J］.环境化学，2014，33(12)：2027-2037.
TIAN Z，ZHANG Y，YANG M，et al.The origin，environmental distribution and potential application of tetracycline resistance gene-tet(X)［J］. Environmental Chemistry，2014，33(12)：2027-2037.(in Chinese)
［16］王垚，吴疆.质粒介导喹诺酮类药物耐药机制的研究现状及进展［J］.西部医学，2015，27(7)：1108-1112.
WANG Y，WU J.Recent research and progress on plasmid-mediated quinolones resistence［J］.Medical Journal of West China，2015，27(7)：1108-1112.(in Chinese)
［17］GUAN X，XUE X，LIU Y，et al.Plasmid-mediated quinolone resistance--current knowledge and future perspectives［J］.J Int Med Res， 2013，41(1)：20-30.

[1]	ZHI Yan, MEI Chen, LIU Zhenyi, WUYUN Gerile, WANG Hongjun, HU Ge. Research Progress on the Virulence Factors of Avibacterium paragallinarum [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4934-4942.
[2]	WANG Jianing, ZHANG Ziqiang, KONG Dejing, FENG Caicai, ZHANG Feike, LIU Yumei. Isolation and Identification of Klebsiella pneumoniae in Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5198-5206.
[3]	LI Lili, CHEN Kaifeng, CHEN Bing, ZHOU Zhouping, WANG Nanwei, QU Xiaoyun, XU Chenggang, LIAO Ming, ZHANG Jianmin. Regulatory Role of STM1827 in the Biofilm Formation and Environmental Stress of Salmonella Typhimurium [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5207-5217.
[4]	TONG Panpan, HUANG Shunmin, WANG Yudan, SHI Xuhui, CHEN Wenxia, SONG Xinlong, ZHANG Yi, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype and Drug Resistance Analysis of Escherichia coli from Diarrhea with Piglets in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 414-420.
[5]	WANG Fei, YANG Jie, Lü Qingjie, WANG Mixue, LIU Peng, ZHANG Ruoyu, SHI Congcong, WANG Xueying, LIN Lin, HUA Lin, SONG Wenbo, LIANG Wan, CHEN Huanchun, WU Bin, PENG Zhong. Isolation and Genomic Characterization of a Meningitis Causing Pasteurella multocida [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4346-4355.
[6]	MEN Kaikai, LIU Jialong, GUO Yage, HE Lei, JIA Yanyan, YU Zuhua. Effects of Chicken TGF-β1 on the Adhesion of Escherichia coli and Salmonella pullorum to DF1 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4000-4007.
[7]	YANG Yang, XIE Liqing, HU Pei, GAO Lixu, YUAN Xiang, LI Pan, PENG Yuanyi, LI Nengzhang. Construction of Bovine Pasteurella multocida Type A hyaD Mutant and Its Cross-protection Analysis in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3582-3597.
[8]	SHAO Ying, FU Dandan, WU Xiaoyan, GU Yi, SONG Xiangjun, TU Jian, QI Kezong. Effect of the ETT2 Structural Gene epaPQR on the Biological Properties and Pathogenicity of Avian Pathogenic Escherichia coli [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3631-3641.
[9]	XU Wenbo, WU Limei, LIU Xin, LI Sheng, HUANG Fushen, LI Runcheng. Analysis on Complete Genome Sequence and Pathogenic Genes of a Pasteurella multocida Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1858-1869.
[10]	WANG Xi, LI Ke, LI Tingcui, YAN Hongya, ZHAO Rong, CHANG Zhishun, LIAO Ming, SUN Minhua, XIN Aiguo. MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1626-1631.
[11]	WANG Jun, LI Jun, CUI Guolin. The Effect of R91S Mutation in FliC on the Flagellar Shape and Salmonella Enteritidis Colonization in BALB/c Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 607-617.
[12]	TONG Panpan, ZHANG Mengmeng, CHEN Wenxia, LIU Luyao, ZHANG Ling, TANG Xuelin, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype, Virulence Genes and Drug Resistance Analysis of Shiga Toxin-producing Escherichia coli from Cattle, Sheep and Camel in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3660-3668.
[13]	CHEN Chaoqun, CHEN Jiali, ZHOU Xuanyi, WU Xue, WANG Jincheng, HUANG Anxiong, HAO Haihong. Establishment of Epidemiological Cut-off Values and Determination of Drug Resistance of Haemophilus parasuis with β-lactam Drugs [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3234-3245.
[14]	HE Zhuolin, TANG Minjia, ZHANG Xuejing, HOU Xiao, PU Wanxia. ERIC-PCR Genotyping and Phylogenetic Grouping of Escherichia coli from a Dairy Farm Environment [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 2059-2064.
[15]	YI Zhengfei, ZHANG Yaodong, WANG Yao, ZHU Hong, TAO Chenglin, AFAYIBO Dossêh Jean Apôtre, LI Tao, QI Jingjing, TIAN Mingxing, DING Chan, YU Shengqing, WANG Shaohui. Two Component System CpxR Contributes to the Antibiotic Resistance, Serum Bactericidal Resistance and Virulence of Avian Pathogenic Escherichia coli [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1053-1060.

Investigation of Antimicrobial Agents Resistance Transferred Horizontally in Fluoroquinolone Resistant Escherichia coli from Swine

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments